Strain Degeneration in White Button Mushroom (Agaricus bisporus) Using Amplified Fragment Length Polymorphism

Background: The growing population and limited agricultural land have pushed the demand for higher food production per area. Edible mushrooms are a rich source of protein. Agaricus bisporus is most common edible mushroom with high economic value. One important steps in process white button spawn. It highly to make pure high-quality mycelium during Studies shown that at this stage production, level morphological changes occurs some mushrooms, which causes formation fluffy-shaped mycelium. This phenomenon kind strain degeneration one biggest concerns producers mushroom. Objectives: purpose study was investigate nature stroma (strain degeneration) mushroom, identify genetic changes. Methods: In experiment, ten successive subcultures were produced from mother on potato dextrose agar medium (pH: 6.2), examined after subcultures. DNA extraction performed sampling mother, normal, fluffy mycelia. Six pairs designed primers propagated parts loaded into six polyacrylamide gels. fragments visualized using silver staining. Genetic comparisons made between mycelia by amplified fragment length polymorphism (AFLP) method, results evaluated POPGENE software. Results: AFLP marker 137 H2 strain, 57 had For 737 149 produced, 73 On average, about 42 49% strains, respectively. degree among samples confirmed differences Conclusions: Based present study, we concluded degeneration, manifests as has origin. These can be attributed diverse factors, such heterokaryosis, asexual recombination, mitosis crossing over. factors their effects require further evaluations.